Details

Autor(en) / Beteiligte

• Quan, Yunlin
• Zhu, Zhixian
• Tang, Dezhi
• Zhu, Shu
• Wang, Cailian
• Chen, Ke
• Ni, Zhonghua

Titel

An ultra-thin silicon nitride membrane for label-free CTCs isolation from whole blood with low WBC residue

Ist Teil von

• Separation and purification technology, 2022-09, Vol.296, p.121349, Article 121349

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• •An ultra-thin Si3N4 membrane was proposed for CTCs isolation from whole blood.•Tumor cells recovery rate greater than 82% and WBC residue dramatically reduced to ∼ 500.•Whole blood without pretreatment was processed at a high throughput of 1 mL/min.•CTCs of cancer patients were detected in only 1 mL peripheral blood. Microfiltration is the most straightforward and effective method to enrich circulating tumor cells (CTCs) from large numbers of background blood cells in the peripheral blood. It is still very challenging to achieve both high recovery of CTCs and low residue of white blood cells (WBCs) through one-time whole blood filtration. In this study, through a simulation, we revealed that reducing the thickness of the filter significantly reduces the WBC residue. A filter device was created using a 400-nm-thick Si3N4 membrane. The recovery rates of A549 and MCF-7 tumor cell lines were 82.04 ± 1.19% and 86.53 ± 3.27%, respectively, while the residual number of WBCs was largely reduced from 6.84 × 106 cells/mL cells to 604 ± 33.74 cells/mL. In addition, the assembled device could process whole blood without pretreatment at a high throughput of 1 mL/min. Utilizing as small volume of peripheral blood as 1 mL, CTCs were enriched by our device in four of six lung cancer patients and four of five breast cancer patients. The lower WBC residue is advantageous in CTC enumeration and subsequent proteomic or genomic characterization. Therefore, this technology enables label-free CTC isolation with a low WBC residue from whole blood, paving the way for a CTC analysis in clinical applications.