Details

Autor(en) / Beteiligte

• Suresh, Patil Shivprasad
• Singh, Prithvi Pal
• Anmol
• Kapoor, Smita
• Padwad, Yogendra S.
• Sharma, Upendra

Titel

Lactic acid-based deep eutectic solvent: An efficient green media for the selective extraction of steroidal saponins from Trillium govanianum

Ist Teil von

• Separation and purification technology, 2022-08, Vol.294, p.121105, Article 121105

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •Lactic-acid-based deep eutectic solvents efficiently extract steroidal saponins from Trilium govanianum.•A total of 21 NADESs were synthesized, choline chloride-lactic acid (1:1; NADES 1) being the most effective among 15 tested NADESs to extract steroidal saponins from Trilium govanianum.•The extraction efficiency of NADES 1 was found to be 53.72 % higher than that of conventional hydroethanolic extraction media.•UHPLC-ELSD method was used for the quantification of seven steroidal saponins in the NADES 1 based extract from Trilium govanianum.•The steroidal saponins-enriched extract obtained by using NADES 1 significantly inhibits acetylcholinesterase activity. Natural deep eutectic solvents have a high solvation and food-grade characteristics, which make them a preffered choice for extracting bioactives to develop nutraceutical and cosmetic formulations. This research aimed to investigate the systematic effects of several NADESs on the ultrasonic-assisted extraction of steroidal saponins from the rhizomes of Trillium govanianum Wall. ex D.Don, which is utilized in traditional Ayurvedic medicine. After screening of fifteen different NADESs, choline chloride: lactic acid (1:1) (NADES 1) was found most effective eutectic solvent for the extraction of steroidal saponins performed by the three-round micro-extraction (1 mL). Compared with hydro-ethanolic solvent-based extraction, lactic acid-derived NADESs showed higher extractability of steroidal saponins. The extraction efficiency of bioactive steroidal saponins e.g. borassoside E and protodioscin was also enhanced with NADES 1. The effects of extraction variables such as S:L ratio, working duration, and temperature on the extraction of steroidal saponins by NADES 1 was also studied and optimized. Furthermore, optimized parameters were validated by performing 100 mL NADES 1-based extraction to recover phytomolecules and NADES 1 by using Diaion HP20 resin column chromatography. As a proof of concept, this study suggested that lactic acid-derived NADESs are effective media for the extraction of steroidal saponins from biomass. The cytocompatibility assay of NADESs with NRK-52E and IEC-6 cells revealed the safe nature of NADESs to normal animal cells. The steroidal saponins-enriched extract obtained with NADES 1 has shown promising antagonistic effects on acetylcholinesterase activity.