Details

Autor(en) / Beteiligte

• Charlie-Silva, Ives
• Feitosa, Natália Martins
• Fukushima, Hirla Costa Silva
• Borra, Ricardo Carneiro
• Foglio, Mary Ann
• Xavier, Rogéria Maura Pazini
• de Melo Hoyos, Daniela Chemin
• de Oliveira Sousa, Ilza Maria
• de Souza, Giovane Galdino
• Bailone, Ricardo Lacava
• de Andrade Belo, Marco Antonio
• Correia, Sostenes Apolo Marcelino
• Junior, José Dias Corrêa
• Pierezan, Felipe
• Malafaia, Guilherme

Titel

Effects of nanocapsules of poly-ε-caprolactone containing artemisinin on zebrafish early-life stages and adults

Ist Teil von

• The Science of the total environment, 2021-02, Vol.756, p.143851, Article 143851

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Artemisinin extracted from Artemisia annua L. plants has a range of properties that qualifies it to treat several diseases, such as malaria and cancer. However, it has short half-life, which requires making continuous use of it, which has motivated the association of artemisinin (ART) with polymeric nanoparticles to increase its therapeutic efficiency. However, the ecotoxicological safety of this association has been questioned, given the scarcity of studies in this area. Thus, in this work the toxicity of Poly (ε-Caprolactone) nanocapsules added with ART (ART-NANO) in zebrafish (Danio rerio), embryos and adults was studied. Different endpoints were analyzed in organisms exposed to ART-NANO, including those predictive of embryotoxicity and histopatoxicity. Embryotoxicity was analyzed based on Organization for Economic Co-operation and Development (OECD) test guideline (236) for fish embryo acute toxicity applied to zebrafish (Danio rerio) at 96 hpf under five nominal logarithmic concentrations (0.125 to 2.0 mg/ L). Our results demonstrate, mainly, that fertilized eggs presented increased coagulation, lack of heart rate, vitelline sac displacement and lack of somite formation. On the other hand, adult individuals (exposed to the same concentrations and evaluated after 24 and 96 h of exposure) have shown increased pericarditis. Therefore, the treatment based on ART, poly (ε-caprolactone) nanocapsules and on their combination at different concentrations have shown toxic effects on zebrafish embryos and adult individuals. [Display omitted]