Details

Autor(en) / Beteiligte

• Jose, Sherin
• K, Mary Mathew
• CS, Anisha
• Sasidharan, Swapna
• YS, Rao

Titel

Development of an ISSR based SCAR marker to identify small cardamom Malabar (prostrate panicle) variety (Elettaria cardamomum (L.) Maton.)

Ist Teil von

• Scientia horticulturae, 2022-02, Vol.294, p.110777, Article 110777

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Small cardamom (Elettaria cardamomum (L.) Maton), is indigenous to the tropical forests of Western Ghats of Southern India.•The cardamom of commerce is the dried fruit (capsule) of the plant.•Malabar, Mysore and Vazhukka are the three natural varieties having prostrate, erect and semi-erect panicles.•Panicle morphology is considered as the key morphological descriptor for this perennial crop.•The developed variety-specific SCAR marker proved to be reliable and efficient in distinguishing small cardamom varieties based on panicle morphology . Small cardamom (Elettaria cardamomum (L.) Maton), a perennial cash crop exhibits an array of natural variations. Malabar, Mysore and Vazhukka are the three major cultivated forms/natural varieties of cardamom, with prostrate, erect and semi-erect panicles respectively. In terms of several qualitative and quantitative features, the Malabar variety is relatively superior to the three varieties. However, the major morphologically discriminative characteristic, panicle orientation, is only visible until the plant has reached physiological maturity. A variety-specific marker was developed and defined in this study for the rapid and accurate differentiation of Malabar varieties at the juvenile stage. In total, 35 ISSR primers were initially screened in 50 small cardamom accessions and two outgroup types, with 18 of them yielding distinct, reproducible profiles. In individuals of the Malabar variety, the ISSR primer (UBC 866–1500 bp) generated an intact, distinct, variety-specific amplicon, which was therefore cloned, sequenced and characterized. Small cardamom accessions from the Malabar variety (prostrate panicle) from different geographic regions, as well as varieties with erect panicles and hybrids, were used to validate the developed SCAR (sequence characterized amplified region) marker(named SBBT4F/SBBT3R). Consistent amplification of a distinct 1350 bp fragment in Malabar variants validated the marker's specificity. According to the findings, the SCAR marker is promising in identifying cardamom varieties with prostrate panicles (Malabar) and thus is expected to make significant contributions in the authentication of varietal selection from F1 hybrids during breeding programmes by reducing the time and labor involved in hybridization experiments in this perennial crop.