Details

Autor(en) / Beteiligte

• Shi, Zhaowan
• Li, Zhaoyang
• Yang, Xueqin
• He, Yanyan
• Wang, Xiaofeng

Titel

Production of endo-β-mannanase, superoxide radicals, hydrogen peroxide, and peroxidase in the micropylar endosperm of pepper seed during germination is determined by the endosperm itself

Ist Teil von

• Scientia horticulturae, 2022-02, Vol.294, p.110757, Article 110757

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Endo-β-mannanase, O2.−, H2O2, and ∙OH production-related peroxidase are tissue-specifically expressed in the micropylar endosperm prior to radicle protrusion during pepper seed germination.•Isolated endosperm-halves incubated alone in the water can still produce endo-β-mannanase, O2.−, H2O2, and peroxidase specifically in the micropylar endosperm.•No endo-β-mannanase, O2.−, H2O2, and peroxidase were induced specifically in the lateral areas of the incubated isolated endosperm-halves touched by small glass beads.•Tissue-specific production of endo-β-mannanase, O2.−, H2O2, and peroxidase in the micropylar endosperm is determined by the endosperm itself. Cell wall-loosening enzymes and non-enzymatic factors have been demonstrated to be expressed specifically in the micropylar endosperm during seed germination, but the reason remains poorly understood. In this study, we used pepper seeds as plant materials to investigate this. At first, we showed that endo-β-mannanase, O2.−, H2O2, and ∙OH production-related peroxidase were tissue-specifically expressed also in the micropylar endosperm prior to radicle protrusion and thus played important roles in seed germination. Next, we incubated isolated de-embryonated seed halves with endosperm and seed coat but without embryo (shorted as endosperm-halves) alone in the water and found that they could still produce endo-β-mannanase, O2∙־, H2O2, and peroxidase specifically in the micropylar endosperm. We also utilized small glass beads to mimic radicles to touch onto the lateral areas of the incubated isolated endosperm-halves but found no endo-β-mannanase, O2∙־, H2O2, and peroxidase were induced specifically in the touched areas. Therefore, we propose that tissue-specific production of endo-β-mannanase, O2∙־, H2O2, and peroxidase in the micropylar endosperm is determined by the endosperm itself.