Details

Autor(en) / Beteiligte

• Liu, Bicheng
• Peng, Zoujun
• Wu, Sihao
• He, Tianshuang
• Qiu, Ping

Titel

A sensitive fluorescent assay for the determination of parathion-methyl using AHNSA probe with MnO2 nanosheets

Ist Teil von

• Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 2021-02, Vol.247, p.119146, Article 119146

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• In the presence of OPs, the activity of AChE was inhibited, accompanied by the restraint of the redox reaction of MnO2 NS, therefore the fluorescence of AHNSA was quenched. [Display omitted] •For the first time, AHNSA was used as the fluorescence probe to detect OPs combined with MnO2 nanosheets.•The method exhibits ultra-sensitivity and high-selectivity toward OPs.•The probe was developed with low cost, simple interpretation and easy handling. In this paper, a novel fluorescence assay has been constructed for the determination of parathion-methyl (PM) by using 4-amino-3-hydroxy-1-naphthalenesulfonic acid (AHNSA) as probe. MnO2 nanosheets (MnO2 NS) could quench the fluorescence of AHNSA, while Mn2+, the reduction product of MnO2 NS, has no influence on it, resulting in fluorescence recovery. This is because that MnO2 NS have oxidized characteristic, and they can react with choline (TCh), which is the product of acetylthiocholine (ATCh) catalyzed by acetylcholinesterase (AChE). In the presence of OPs, the activity of AChE was inhibited, accompanied by the restraint of the redox reaction of MnO2 NS, therefore the fluorescence of AHNSA was quenched. Under the optimized experimental conditions, a linear range of PM was determined to be 0.4–40 ng/mL (R2 = 0.997) by the proposed method with the limit of detection for 0.18 ng/mL (S/N = 3). The assay was successfully applied to the determination of PM in lake water, which average recoveries were between 86.5% and 114.4%.