Details

Autor(en) / Beteiligte

• Murray, Byron K.
• Brown, Brita
• Scherer, Philip M.
• Tomer, David P.
• Garvin, Kaedi R.
• Hughes, Bronwyn G.
• O'Neill, Kim L.

Titel

Induction of apoptosis in HT-29 human colon adenocarcinoma cells by 13- cis-retinoic acid and vitamin E succinate

Ist Teil von

• Nutrition research (New York, N.Y.), 2006-04, Vol.26 (4), p.186-192

Ort / Verlag

New York, NY: Elsevier Inc

Erscheinungsjahr

2006

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Recent studies have shown that retinoids and vitamin E are effective growth inhibitors of numerous cancer cell lines. In the present study, vitamin A derivative 13- cis-retinoic acid and α-tocopherol succinate were administered to HT-29 human colon adenocarcinoma cells individually or in combination for a period of 8 days. Cells treated with individual compounds for 8 days attained 80% of the control population. Cells treated with both compounds reached 25% of the control population after 4 days and less than 1% of the control population after 8 days. After 4 days, treated cells became cytostatic, with more than 50% of the cells detaching by day 8. This synergistic effect was specific only for HT-29 cells as compared with normal human colon cells. Treatment resulted in morphological and biochemical changes indicative of apoptotic cell death, including cell cycle blockage at the G 1/S interphase, chromatin condensation, nuclear fragmentation, DNA damage, and caspase 3 activation.