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A highly sensitive capacitance aptamer/PEGferrocene/AuNPs based platform for the sensing of aflatoxin M1toxin in cow milk.
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•Simple method to elaborate the AFM1 biosensoor.•Aptamer covalent attachment to a PEG/ferrocene derivative for efficient detection.•Selective and sensitive detection of AFM1.•The nanoaptasensor directly detect AFM1 in milk.
Detection of ultra-trace amounts of aflatoxin M1 (AFM1) is an important requirement for food safety since it is a toxic mycotoxin, present in cow milk, with strictly regulated low levels. To achieve detection of low levels of AFM1, we developed a new nanometric aptasensing platform based on the modified SPCE, which was decorated with AuNPs, a tetraethylene glycol ferrocene derivative and an anti-AFM1 aptamer. The ferrocene tethered to AuNPs served as a capacitance transducer contributing to an interfacial charge. The capacitive signal was used to quantify the toxin. This study revealed a good sensitivity toward the toxin with a dynamic range of 20 to 300 pg·mL−1 and a LOD as low as 7.14 pg·mL−1 (S/N = 3). The nanoaptosensor exhibits very good metrological performances. To evaluate the sensing platform, the latter was applied to detect the presence of AFM1 in pasteurized cow milk.