Details

Autor(en) / Beteiligte

• Sánchez-Paniagua López, Marta
• López-Ruiz, Beatriz

Titel

Electrochemical biosensor based on ionic liquid polymeric microparticles. An analytical platform for catechol

Ist Teil von

• Microchemical journal, 2018-05, Vol.138, p.173-179

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A catechol amperometric biosensor based on tyrosinase has been successfully developed. Tyrosinase was immobilized into microparticles prepared by polymerization of the ionic liquid 1-vinyl-3-ethyl-imidazolium bromide (ViEtIm+Br−). Different kinds of microparticles with entrapped tyrosinase based on ionic liquid were obtained from the latter, by a simple anion-exchange reaction. The synthesis of the microparticles was optimized. The analytical method based on the proposed device was also optimized in terms of pH and temperature. Optimal values were 6.5 and 25 °C, respectively. The characterization of both, microparticles and analytical device, was performed by cyclic voltammetry and electrochemical impedance spectroscopy. A linear relationship between the current signal and the catechol concentration was obtained in the range from 3.9·10−5 to 2.5·10−4 M, with a sensitivity of 17.95 mA M−1 cm−2 and a detection limit of 20 μM. The tyrosinase activity of lyophilized poly(ViEtIm+Br−) microparticles was unchanged for at least 9 months after their synthesis. The biosensor maintains 95% of the initial response 21 days after its first use. The biosensor has been applied for detecting phenol compounds in wastewater samples. The recoveries were found in the range of 91–96%. [Display omitted] •Polymer microparticles based on ionic liquid has been applied as immobilization matrices for tyrosinase biosensor.•A great stability of the enzyme immobilized into the polymer network is obtained, at least 9 months after their synthesis•The most remarkable characteristics of this analytical device are its easy preparation and high stability•The biosensor was applied to tyramine detection in waste water from an olive oil refinement with recoveries of 91-96 %