Details

Autor(en) / Beteiligte

• Liu, Junyan
• Huang, Tengyi
• Mao, Yuzhu
• Soteyome, Thanapop
• Liu, Gongliang
• Seneviratne, Gamini
• Kjellerup, Birthe V.
• Xu, Zhenbo

Titel

Development and application of multiple polymerase spiral reaction (PSR) assays for rapid detection of methicillin resistant Staphylococcus aureus and toxins from rice and flour products

Ist Teil von

• Food science & technology, 2023-01, Vol.173, p.114287, Article 114287

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Methicillin-Resistant Staphylococcus aureus (MRSA) is an important foodborne pathogen and produces a variety of toxins causing serious illnesses. This study aimed at developing a rapid detection assay for MRSA and toxins targeting on two housekeeping genes (mecA and femA) and 5 virulence factors (sea, seb, sec, see and pvl) using the polymerase spiral reaction (PSR), compared with PCR. For naked-eye result inspection, calcein was added and the PSR reaction can be completed at 65ᵒC within 60 min. The PSR assay was subjected to optimization, evaluation, and application in 3 different types of food sample made from rice and flour, designated rice and flour product. The limit of detection of PSR assay for the mecA, femA, sea, seb, sec, see and pvl genes was 4.2 pg/μL, 0.42 pg/μL, 630 pg/μL, 63 pg/μL, 12.3 pg/μL, 1.015 pg/μL and 53 pg/μL, respectively, which was higher than the sensitivity of PCR. In rice and flour products, the detection limit of PSR assay was 104 CFU/mL for mecA, and 103 CFU/mL for femA, sea, seb, sec, see and pvl genes. In conclusion, optimized PSR assay serves as an efficient tool for rapid, cost-effective and accurate testing of MRSA and is suitable for point-of-care detection. •The optimized PSR assay is efficient for rapid detection of MRSA and its toxins (within 65ᵒC for 60 min).•The PSR assay is applied in 3 different food types with high specificity (100%) and sensitivity (10–1000 times > PCR).•By adding calcein, color change from orange to green enables naked-eye inspection, avoiding false positive detection.