Details

Autor(en) / Beteiligte

• Liu, Xiaocui
• Zhang, Zhicai
• Xu, Ling
• Shi, Wenjing
• Sun, Quanshan
• Ding, Chongyang
• Zheng, Huihua
• Wang, Feng

Titel

Dioscorea saponin transforms the structure of truffle exo-polysaccharide and enhances its antioxidant activity

Ist Teil von

• Food science & technology, 2020-06, Vol.127, p.109417, Article 109417

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Dioscorea saponin (DS) can increase the exo-polysaccharide production of Tuber melanosporum. In the present study, we assessed the effect of DS on the structure and antioxidant activity of exo-polysaccharide. In the absence of DS, only one exo-polysaccharide (TP1) composed of mannose, rhamnose and glucose was isolated. However, two exo-polysaccharides (STP1 and STP2) were isolated in the presence of DS. STP1 was composed of glucose, galactose and arabinose, and STP2 consisted of rhamnose, glucose, galactose and arabinose. The skeletal structure of TP1, STP1 and STP2 was composed of α-d-glucopyranose. The antioxidant activity of exo-polysaccharide exhibited a concentration-dependent manner. The DPPH radical-scavenging rate (61.37%) and total antioxidant activity (50.02%) of STP were higher compared with TP1. The STP increased the DPPH radical-scavenging rate depending on the increase of the maximum rate of the reaction at saturating substrate concentrations (kcat), while STP increased the reducing power depending on the kcat and affinity of substrate to STP. The inhibitory action of STP on hydroxyl radical generation depended on the blockage of Fe2+ by STP, making it unable to react with H2O2 to form ·OH, and STP could bind to the Fe2+- H2O2 complex and prevent the Fe2+ from turning H2O2 into product. •DS can transforms the structure of truffle EPS.•DS can enhances its antioxidant activity of truffle EPS.•The increase of DPPH radical-scavenging rate depended on μmax increase.•STP increased the total reducing power depending on the μmax and Km.•The inhibitory action of STP on hydroxyl radical generation was a mixed inhibition.