Details

Autor(en) / Beteiligte

• Fang, Huajuan
• Zhang, Xingping
• Ma, Liyuan
• Xiao, Yao
• Yang, Hualin
• Zhou, Yu

Titel

Fluorescence detection of glyphosate based on G-quadruplex and porphyrin metalation

Ist Teil von

• Journal of food composition and analysis, 2023-03, Vol.116, p.105074, Article 105074

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• In this paper, a fluorescence biosensor was developed for detection of glyphosate (GLYP) based on G-quadruplex (G4) and porphyrin metalation. In this strategy, G4 was utilized to increase the fluorescence of N-methylmesoporphyrin IX (NMM) and to catalyze the insertion of Cu2+ into NMM. Because Cu2+-NMM had no fluorescence, the fluorescence of NMM was quenched by Cu2+ in the absence of GLYP. However, in the presence of GLYP, it can bind to Cu2+ to inhibit the NMM metalation and the fluorescence was recovered. This GLYP biosensor did not require any modification and can complete the detection process in 23 min. To our knowledge, this was the first time using the porphyrin metalation to detect GLYP. The limits of detection (LOD) of this GLYP biosensor in water, rice, maize, and wheat samples were 0.065 μΜ, 0.13 μM, 0.14 μM, and 0.27 μM, respectively. Meanwhile, this GLYP biosensor can selectively identify GLYP among organophosphorus pesticides. The satisfactory recovery rates in agricultural products (88.0–103%) suggested this biosensor had great application prospects for measuring GLYP. •Porphyrin metalation was used to design glyphosate biosensor.•This biosensor can selectively identify GLYP among organophosphorus pesticides.•This biosensor was sensitive in both water samples and agricultural products.•This biosensor can complete the detection process in 23 min and did not require synthetic materials.