Details

Autor(en) / Beteiligte

• Silva, A.F.V.
• Santos, L.A.
• Valença, R.B.
• Porto, T.S.
• Da Motta Sobrinho, M.A.
• Gomes, G.J.C.
• Jucá, J.F.T.
• Santos, A.F.M.S.

Titel

Cellulase production to obtain biogas from passion fruit (Passiflora edulis) peel waste hydrolysate

Ist Teil von

• Journal of environmental chemical engineering, 2019-12, Vol.7 (6), p.103510, Article 103510

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •Utilization of passion fruit peel residues to obtain cellulases by submerged fermentation.•Cellulase production for enzymatic hydrolysis as a pre-treatment for obtaining energy sources.•Obtaining and potentiating of production biogas through enzymatic hydrolysate of passion fruit peel.•The two hydrolysates were satisfactorily adjusted to the three models studied. Enzyme usage for valorization of organic and agroindustrial wastes is a biotechnology field that had been increasing due to economic and environmental benefits that add to productive cycles. Enzymatic hydrolysis is one of the possible pre-treatments used with the aim of facilitating lignocellulosic waste biodigestion. This work evaluated cellulase production by Aspergillus japonicus URM5620 and its use as enzymatic pre-treatment on passion fruit peel waste (Passiflora edulis) in order to facilitate the anaerobic biodigestion for biogas generation purposes. Cellulases were produced by submerged fermentation, being maximized using a factorial 2² design, analysing substrate concentration (passion fruit peel) (1.0 to 3.0%) and glucose (0.5 to 1.0%). Enzymatic hydrolyzate obtained from submerged fermentation was biodigested in anaerobic reactor, in a laboratory scale. The highest activities of 1.2 U/mL total cellulase (FPase) and 1.7 U/mL endoglucanase (CMCase) were obtained at 3% of substrate and 1% of glucose. Biogas production from hydrolyzed waste provided methane with a concentration of above 64% by volume after 15 days. Fermentation pre-treatment, using Aspergillus japonicus, was a step that potentially facilitates and accelerates lignocellulosic organic waste biodigestion.