Details

Autor(en) / Beteiligte

• Shinde, Ujwala A.
• De, Abhijit
• Godse, Rutika D.
• Rathod, Maitreyi
• Thorat, Rahul
• Sharma, Nisha
• Ansari, Mudassir
• Singh, Kavita

Titel

Efficacy evaluation of carboplatin -etoposide Co-loaded PLGA microparticles for subconjunctival chemotherapy in retinoblastoma

Ist Teil von

• Journal of drug delivery science and technology, 2024-02, Vol.92, p.105333, Article 105333

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Systemic chemotherapy for retinoblastoma comes with various haematological side effects, hence, require newer treatment approaches. This study developed carboplatin (CPT) and etoposide (ETP) loaded biodegradable PLGA microparticles as a combination therapy for extended action decreasing frequency of administration. CPT and ETP loaded PLGA microparticles (CE-MIP) were prepared by emulsification-spray drying technique. The optimized drug loaded PLGA microparticles were characterized for particle size (3–14 μm), zeta potential (−23.0 to −34.2 mV), entrapment efficiency (ETP - >80 % & CPT - >60 %) and in vitro release (CPT – 4 h & ETP – 72 h). In vitro cytotoxicity studies of CE-MIP showed greater anti-cancer effect (25.33 % cell viability) compared to plain drug mixture (38.56 % cell viability) in Y-79 cells. In WERI-Rb cell lines, the CE-MIP and plain drug mixture at 100 μM showed percent cell viability as 37.82 % and 66.08 % respectively. In the soft agar assay, the CE-MIP treatment demonstrated a significant reduction in the number of colonies (∼50 % reduction) formed compared to control. In orthotropic mouse model of retinoblastoma, the apoptotic effect of subconjunctival CE-MIP (15.7 %) was greater compared to control (7.9 %). Mean tumor burden of CE-MIP treated eyes was significantly smaller compared to the untreated control eyes showing the anti-tumor efficacy of developed CE-MIP. [Display omitted] •Retinoblastoma (RB) is a primary intraocular malignancy of childhood.•Subconjunctival Carboplatin-etoposide coloaded PLGA microparticles in management of RB.•Time and dose dependent cytotoxicity on Y79 & WERI-Rb-1 cell lines.•Soft agar colony formation assay confirmed antitumorigenic potential.•Remarkable tumor suppression in xenograft mouse model.