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Different mechanisms control lipoxygenase activity in durum wheat kernels
Ist Teil von
Journal of cereal science, 2010-09, Vol.52 (2), p.121-128
Ort / Verlag
Kidlington: Elsevier Ltd
Erscheinungsjahr
2010
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals Complete
Beschreibungen/Notizen
Yellow pigment content (YPC), lipoxygenase (LPX) activity and the polymorphism at the
Lpx-B1.1 locus were assessed on an Italian durum wheat germplasm collection and four varieties contrasting for YPC and LPX activity were then characterized in terms of
Lpx gene expression, biochemical LPX properties and their performance during pasta processing. The screening of 71 genotypes showed a great genetic variability for YPC (3.68–9.43
μg/g
dw) and LPX activity (0.02–7.91
EU/g
dw). The
Lpx-B1.1 polymorphism was significantly associated with differences in LPX activity. Besides the
Lpx-B1.1 deletion, different expression levels of
Lpx mRNAs were found associated with differences in LPX activity. The temporal expression of three
Lpx genes showed different profiles among the cultivars investigated. Genotypes with high LPX activity showed an anticipated temporal expression for
Lpx-1 compared to low LPX activity ones. The LPX activity in the analysed genotypes is most likely due to the contribution of different LPX isoforms observed in the later stage of grain filling.