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Valorisation of slaughter house and deinking paper waste streams for the production of enzyme by Trichoderma reesei
Ist Teil von
Journal of cleaner production, 2020-12, Vol.275, p.122882, Article 122882
Ort / Verlag
Elsevier Ltd
Erscheinungsjahr
2020
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
The study investigates for the first time the possibility of using carbon rich paper recovery sludge, and nitrogen rich meat processing industry waste as cultivation medium for the production of high value enzymes needed in the respective industries. The complex cellulose rich deinking sludge was able to support the growth of many industrially relevant enzyme producing microorganisms (Bacillus licheniformis, Candida cylindracea, Aspergillus oryzae, Trichoderma reesei) and of recombinant enzyme producers (Escherichia coli and Pichia pastoris). Further detailed studies with Trichoderma reesei as model organism demonstrated that the organism was able to grow optimally in the presence of 40gL-1 paper sludge as carbon source and 67.5 gL-1 pasteurised blood as nitrogen source substituted in Mandels medium. Under these conditions cellulase activities up to 28.1 nkat FPU were achieved. Anyhow, to achieve these results pretreatment of both waste streams is inevitable. In summary, this study provides the practical basis for a valorisation systems of paper industry waste to produce valuable enzymes to be used on-site in paper processing or for other purposes.
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•Blood and paper waste used for microorganism growth.•Growth and cellulase production achieved utilising blood waste streams.•Pretreatment of paper waste and blood waste is necessary.•Waste to Value with in house use of cellulase in paper industry.