Details

Autor(en) / Beteiligte

• Nikolaev, Viacheslav O., PhD
• Boivin, Valérie, PhD
• Störk, Stefan, MD, PhD
• Angermann, Christiane E., MD
• Ertl, Georg, MD
• Lohse, Martin J., MD
• Jahns, Roland, MD

Titel

A Novel Fluorescence Method for the Rapid Detection of Functional β1 -Adrenergic Receptor Autoantibodies in Heart Failure

Ist Teil von

• Journal of the American College of Cardiology, 2007-07, Vol.50 (5), p.423-431

Ort / Verlag

New York, NY: Elsevier Inc

Erscheinungsjahr

2007

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• A Novel Fluorescence Method for the Rapid Detection of Functional β1 -Adrenergic Receptor Autoantibodies in Heart Failure Viacheslav O. Nikolaev, Valérie Boivin, Stefan Störk, Christiane E. Angermann, Georg Ertl, Martin J. Lohse, Roland Jahns Activating autoantibodies targeting the β1 -adrenoceptor may play a pathophysiological role in heart failure, but there is no reliable method for their detection. Here, we present a new method based on cyclic adenosine monophosphate (cAMP) measurements using a novel fluorescence sensor (Epac1-camps). Significant cAMP signals were induced by approximately 73% of immunoglobulin G fractions prepared from 55 dilated cardiomyopathy patients, 33% of them representing “high” activators (blocked by second extracellular β1 -receptor loop peptides), the other 40% “low” activators (blocked by first extracellular β1 -receptor loop peptides). In ischemic cardiomyopathy patients, we detected 5 high but no low activators. The immunoglobulin G prepared from 50 healthy subjects produced no cAMP signals. Our method facilitates rapid detection of different β1 -autoantibody classes and evaluation of novel therapeutic strategies.