Details

Autor(en) / Beteiligte

• Liu, Pu
• Zhang, Yu
• Gao, Jia-yu
• Du, Ming-zhu
• Zhang, Kai
• Zhang, Jiang-lei
• Xue, Nan-chuan
• Yan, Mi
• Qu, Chun-xiao
• Deng, Rui-xue

Titel

HPLC-DAD analysis of 15 monoterpene glycosides in oil peony seed cakes sourced from different cultivation areas in China

Ist Teil von

• Industrial crops and products, 2018-08, Vol.118, p.259-270

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •HPLC-DAD method to analyse 15 monoterpene glycoside (MG) was developed.•The effects of geographical differences on the content of MG were evaluated.•Oil peony (OP) seeds could be clustered into three groups based on MG content.•Classification of OP was associated to the geographical location of cultivation area. The oil peony seed cake, one of the most important by-products during the seed oil preparation, contains numerous monoterpene glycosides. The development and optimization of a simple and reproducible method for simultaneous qualitative and quantitative determination of monoterpene glycosides from oil peony seed cakes would be a valuable work for its further utilization. In this study, an HPLC method using DAD detector was developed to analyse fifteen monoterpene glycosides in oil peony seed cakes. The optimum resolution of the fifteen monoterpene glycosides was achieved on an ODS-AQ column. Acetonitrile (solvent A) and 0.5% potassium dihydrogen phosphate solution (pH 2.8) (solvent B) were used as the mobile phase by a gradient elution. Paeoniflorin showed the highest content amongst 15 monoterpene glycosides. It accounted for more than half of the total content of the fifteen monoterpene glycosides (TCFMG) in oil peony seed cake, followed by oxypaeoniflorin and albiflorin. The samples of different cultivation areas contain the different contents of monoterpene glycosides, mainly determined by their geographical variation. The higher level contents of monoterpene glycoside are believed to have more potential in medicinal application. The method thus could be used to control the quality of products from oil peony seed cakes.