Details

Autor(en) / Beteiligte

• Manca, Maria Letizia
• Marongiu, Francesca
• Castangia, Ines
• Catalán-Latorre, Ana
• Caddeo, Carla
• Bacchetta, Gianluigi
• Ennas, Guido
• Zaru, Marco
• Fadda, Anna Maria
• Manconi, Maria

Titel

Protective effect of grape extract phospholipid vesicles against oxidative stress skin damages

Ist Teil von

• Industrial crops and products, 2016-05, Vol.83, p.561-567

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2016

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •Grape pomaces were used to obtain an extract rich in polyphenols.•The extraction was performed homogenizing the biomass in an ethanol/water mixture.•The extract was incorporated in liposomes and innovative modified vesicles (PEVs).•The extract-loaded vesicles protected keratinocytes and fibroblasts against H2O2 damage. Grape extract rich in polyphenols (∼129±32mg of gallic acid equivalents per g of dry extract) was obtained from the pomaces of Cannonau grapes by homogenization in an ethanol/water mixture. The efficacy of ultrasounds in speeding up the extraction kinetics of polyphenols was demonstrated. The extract was incorporated in liposomes and PEVs (penetration enhancer containing vesicles) with Labrasol® or Labrasol®/ethanol. All the vesicles were spherical and predominantly unilamellar: liposomes were large (∼927nm) and polydispersed (PI ∼0.56), while PEVs were small (∼140nm) and fairly homogeneous (PI ∼0.3). Moreover, PEVs were able to incorporate a high amount of the extract (∼98% of the extract used for their preparation, 50mg/ml). The formulations were highly cytocompatible and were able to promote the proliferation of keratinocytes and fibroblasts. In addition, thanks to their antioxidant activity, grape extract formulations provided a cytoprotective effect against oxidative stress damage. Therefore, an efficient, environmentally-friendly extraction strategy is proposed to obtain an extract with high phenolic content from waste grape pomaces, which was incorporated in vesicular systems to maximize the antioxidant power in a cell model of oxidative stress.