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Development of a multiplex PCR assay to discriminate native Hymenoscyphus albidus and introduced Hymenoscyphus fraxineus in Britain and assess their distribution
Ist Teil von
Fungal ecology, 2016-10, Vol.23, p.79-85
Ort / Verlag
Elsevier Ltd
Erscheinungsjahr
2016
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Two Hymenoscyphus species are associated with ash (Fraxinus) in Europe; the recently arrived ash dieback pathogen Hymenoscyphus fraxineus and apparently native Hymenoscyphus albidus. However, in parts of Europe invasive H. fraxineus appears to be replacing H. albidus which may, therefore, face extinction. We designed primers based on calmodulin sequences for use in singleplex and multiplex PCR assays to detect and discriminate between these two morphologically very similar species. The multiplex assay was tested against 50 isolates and 26 apothecial field samples of H. albidus/H. fraxineus from Europe and Asia, plus isolates of Hymenoscyphus fructigenus, Hymenoscyphus koreanus and Hymenoscyphus occultus. Use of the assay confirmed both H. albidus and H. fraxineus were present across southern Britain between 2014 and 2015, with H. albidus detected at eight geographically distinct sites. The sensitivity, specificity and potential applications of the multiplex assay are discussed.
•Native Hymenoscyphus albidus and introduced Hymenoscyphus fraxineus are associated with Fraxinus excelsior.•A multiplex PCR assay was developed for the dual detection of both fungi.•The assay reveals H. albidus as widespread in southern Britain in 2014–15.•Assay uses include ecological monitoring of native H. albidus as the ash dieback pathogen H. fraxineus invades its habitat.