Details

Autor(en) / Beteiligte

• Xing, Ran-Ran
• Hu, Ran-Ran
• Wang, Nan
• Zhang, Jiu-Kai
• Ge, Yi-Qiang
• Chen, Ying

Titel

Authentication of sea cucumber products using NGS-based DNA mini-barcoding

Ist Teil von

• Food control, 2021-11, Vol.129, p.108199, Article 108199

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Sea cucumbers are widely consumed in Asia. This study developed a novel DNA mini-barcode mitochondrial cytochrome c oxidase (COI) of 257 bp to discriminate sea cucumbers species in both raw and processed forms. We designed and compared four mini-barcoding primer sets based on PCR amplification rates against 40 authenticated sea cucumber species. The top-performing primer set was successful for most tested sea cucumber varieties. We also tested the DNA mini-barcode COI in 12 highly processed sea cucumber products using both Sanger sequencing and next-generation DNA sequencing (NGS). This is the first report of highly processed sea cucumber products that detected fraudulent replacement of sea cucumber by one or a blend of species using the NGS approach. The established DNA mini-barcoding system revealed that 83.33% (10/12) of highly processed sea cucumber products were mislabeled. This study identified a need for more regulation of the sea cucumber trade and suggested that DNA mini-barcoding combined with NGS could complement existing sea cucumber identification strategies. •Novel COI mini-barcode (257 bp) was developed to identify sea cucumber species.•Combination of mini-barcode with next generation sequencing for authenticating processed sea cucumber products.•DNA mini-barcoding system is effective in authenticating raw and processed sea cucumber products.•Detection of mislabeling in 83.33% of highly processed sea cucumber products.