Details

Autor(en) / Beteiligte

• Li, Xiao
• Wei, Zeliang
• Wang, Xingyue
• Duan, Feixia
• Xiong, Lidan
• Li, Jingwen
• Tian, Jing
• Jia, Lirong
• Gao, Hong

Titel

Premna microphylla Turcz leaf pectin exhibited antioxidant and anti-inflammatory activities in LPS-stimulated RAW 264.7 macrophages

Ist Teil von

• Food chemistry, 2021-07, Vol.349, p.129164, Article 129164

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2021

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• •RG I, high- and low-methoxyl HG pectin were extracted from P. microphylla Turcz (PMT) leaves.•PMT leaf pectins extracted at different pH were all in amorphous form.•PMT leaf pectins showed radical scavenging effect.•PMT leaf RG I pectin reduced intracellular ROS by activating endogenous antioxidant enzymes.•PMT leaf RG I pectin inhibited inflammatory cytokines in macrophages. Premna microphylla turcz leaf juice with polysaccharides (PMPs) as its main component, are raw material of jelly-like Chinese traditional food “Guanyin tofu”, which were also experiencedly used to relieve inflammation-related symptoms. Here three kinds of PMPs were extracted in alkaline (APMP), water (WPMP) and acidic (HPMP) conditions, being characteristic of RG I, high- and low-methoxyl HG pectin, respectively, in amorphous form with diverse surface microstructures, among which APMP predominantly composed of Glucose instead of galacturonic acid, showing wider molecular weight distribution and more branched chains. PMPs showed remarkable radical scavenging capability, and especially APMP at concentrations above 50 μg/mL effectively inhibited the reactive oxygen species and malondialdehyde production in LPS-stimulated RAW 264.7 macrophages, by enhancing enzymatic activities of endogenous superoxide dismutase, glutathione peroxidase and catalase, and accordingly alleviated inflammatory cytokines. Thus, PMPs could be promising non-toxic natural dietary supplement to improve chronic inflammation-induced diseases.