Details

Autor(en) / Beteiligte

• Zhuang, Xinbo
• Han, Minyi
• Jiang, Xiping
• Bai, Yun
• Zhou, Hengyue
• Li, Chunbao
• Xu, Xing-lian
• Zhou, Guang-hong

Titel

The effects of insoluble dietary fiber on myofibrillar protein gelation: Microstructure and molecular conformations

Ist Teil von

• Food chemistry, 2019-03, Vol.275, p.770-777

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2019

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] •The pure MP gelation had a loose and dispersed network with interconnected water channels.•The MP gelation was significantly improved by IDF addition.•IDF addition eliminate interconnected water channels in the gelation network.•The IDF addition promoted to the protein aggregation during thermal process. The effects of insoluble dietary fiber (IDF) on heat-induced gelation properties, such as microstructural changes and the molecular conformation of myofibrillar proteins (MP), were analyzed by image analyses and Raman spectroscopy. Scanning electron microscopy revealed that pure MP gelation contained a loose and dispersed network with interconnected water channels. With IDF addition, the lacunarity and the particle size of water pores both significantly decreased (P < 0.05) and fractal dimension significantly increased (P < 0.05), which indicated the formation of a homogenous and compact three-dimensional network. Through Raman spectra, IDF addition resulted in modification of amide I and III regions, by a significant decrease in α-helix content, accompanied by an increase of β-sheets, β-turns, and random coil content (P < 0.05); the intensity of CU+005CH stretching vibrations bending vibrations bands were significantly decreased (P < 0.05). Principal component analyses showed significant correlations between textual property, microstructure and molecular conformations. Cluster analyses indicated IDF improved the functionality of MP gel by 1): IDF stable the moisture phase 2) “concentrated” MP promoted structural integrity and compact gelation.