Details

Autor(en) / Beteiligte

• Farcasanu, Ileana C.
• Mitrica, Radu
• Cristache, Ligia
• Nicolau, Ioana
• Ruta, Lavinia L.
• Paslaru, Liliana
• Comorosan, Sorin

Titel

Optical manipulation of Saccharomyces cerevisiae cells reveals that green light protection against UV irradiation is favored by low Ca 2+ and requires intact UPR pathway

Ist Teil von

• FEBS letters, 2013-11, Vol.587 (21), p.3514-3521

Erscheinungsjahr

2013

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Optical manipulation of Saccharomyces cerevisiae cells with high density green photons conferred protection against the deleterious effects of UV radiation. Combining chemical screening with UV irradiation of yeast cells, it was noted that the high density green photons relied on the presence of intact unfolded protein response (UPR) pathway to exert their protective effect and that the low Ca 2+ conditions boosted the effect. UPR chemical inducers tunicamycin, dithiotreitol and calcium chelators augmented the green light effect in a synergic action against UV‐induced damage. Photo‐manipulation of cells was a critical factor since the maximum protection was achieved only when cells were pre‐exposed to green light. Green light provided protection against the deleterious effects of UV irradiation upon yeast cells. High density green photons protected the yeast cells against UV in synergism with unfolded protein response inducers. Photoprotective action of green light against UV required intact UPR pathway. The cnb1Δ cells, lacking the regulatory subunit of calcineurin, became more sensitive to UV after green light exposure.