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A one-step method has been developed for incorporating ss DNA into a bilayer lipid membrane (BLM) on agar electrode, which displayed good responses to complementary ss DNA. The 5′-terminal phosphated end of ss DNA formed a phosphormidate bond with the amino group of
N,
N-diethylamidobenzene (NDAB) in the BLM forming solution. The method is easy to perform and has good reproducibility. Fourier transform infrared spectroscopy (FTIR) showed that the ss DNA was incorporated into the BLM.