Details

Autor(en) / Beteiligte

• Zhang, Cheng
• Hao, Qingkai
• Zhang, Sisheng
• Zhang, Zhengyi
• Zhang, Xianghui
• Sun, Peng
• Pan, Hongyu
• Zhang, Hao
• Sun, Fengjie

Titel

Transcriptomic analysis of Chlorimuron-ethyl degrading bacterial strain Klebsiella jilinsis 2N3

Ist Teil von

• Ecotoxicology and environmental safety, 2019-11, Vol.183, p.109581, Article 109581

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Chlorimuron-ethyl is a sulfonylurea herbicide with a long residual period in the field and is toxic to rotational crops. Klebsiella jilinsis 2N3 is a gram-negative bacterium that can rapidly degrade Chlorimuron-ethyl. In this study, the gene expression changes in strain 2N3 during degradation of Chlorimuron-ethyl was analyzed by RNA-Seq. Results showed that 386 genes were up-regulated and 453 genes were down-regulated. KEGG pathway enrichment analysis revealed the highest enrichment ratio in the pathway of sulfur metabolism. On the basis of the functional annotation and gene expression, we predicted that carboxylesterase, monooxygenase, glycosyltransferase, and cytochrome P450 were involved in the metabolism of Chlorimuron-ethyl biodegradation. Results of qRT-PCR showed that the relative mRNA expression levels of these genes were higher in treatment group than those in control group. The cytochrome P450 encoded by Kj-CysJ and the alkanesulfonate monooxygenase encoded by Kj-SsuD were predicted and further experimentally confirmed by gene knockout as the key enzymes in the biodegradation process. Cultured in basal medium containing Chlorimuron-ethyl (5  mg L−1) in 36 h, the strains of ΔKj-CysJ, ΔKj-SsuD, and WT reached the highest OD600 values of 0.308, 0.873, and 1.085, and the highest degradation rates of Chlorimuron-ethyl of 11.83%, 96.21%, and 95.62%, respectively. •Transcriptome of Chlorimuron-ethyl degrading bacterium Klebsiella jilinsis 2N3.•386 up-regulated and 453 down-regulated genes in degradation of Chlorimuron-ethyl.•KEGG analysis identified ‘Sulfur Metabolism’ with the highest enrichment ratio.•Carboxylesterase and glycosyltransferase are involved in the biodegradation.•Cytochrome P450 and alkanesulfonate monooxygenase are key enzymes in degradation.