Details

Autor(en) / Beteiligte

• Menestrina, Fiorella
• Ronco, Nicolás R.
• Castells, Cecilia B.

Titel

Enantioselective gas chromatography with functionalized cyclodextrins as chiral selectors. Fundamentals of the measurement of absolute association constants using capillary columns

Ist Teil von

• Journal of Chromatography A, 2016-10, Vol.1467, p.482-489

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2016

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• •We propose a reliable GC method for determining enantiomeric association constants.•Capillary GC columns with a g-CD derivative as a chiral selector were constructed.•Absolute association constants of amino acid derivatives with a g-CD were obtained.•True enantioselectivity and apparent enantioselectivity are critically discussed. Chiral capillary GC columns containing different amounts of octakis(6-O-tert-butyldimethylsilyl-2,3-di-O-acetil)-γ-cyclodextrin as chiral selector dissolved in a polymeric matrix were constructed with the aim of determining enantiomeric association constants between a group of well resolved chiral N-trifluoroacetyl amino acid methyl esters and this specific selector at different temperatures. The most relevant sources of uncertainties in the experimental data (hold-up and retention times, and column phase ratios at each temperature) were assessed. These cyclodextrin-based columns are known to enantioseparate a wide variety of chemical compounds, thus, the measurement of the absolute enantioselective constants of a group of solutes with this selector can be useful for systematic studies aimed to a general understanding about how these selectors work. These absolute association constants were estimated from data collected from very simplified experimental systems, and by using the fundamental gas-liquid chromatography equations.