Details

Autor(en) / Beteiligte

• Lu, Jun
• Zhang, Peng-yang
• Li, Ping
• Xie, Jian-wei
• Wang, Jia-bin
• Lin, Jian-xian
• Chen, Qi-yue
• Cao, Long-long
• Huang, Chang-ming
• Zheng, Chao-hui

Titel

Circular RNA hsa_circ_0001368 suppresses the progression of gastric cancer by regulating miR-6506–5p/FOXO3 axis

Ist Teil von

• Biochemical and biophysical research communications, 2019-04, Vol.512 (1), p.29-33

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2019

Quelle

ScienceDirect

Beschreibungen/Notizen

• Gastric cancer (GC) is still a major aggressive malignancy worldwide. While the importance of circular RNAs (circRNAs) involved in carcinogenesis has gradually been acknowledged, their role in human cancers is not largely understood, including in GC. Here, we focused on hsa_circ_0001368 in GC, a novel circRNA that has not been previously reported. In the current study, we found a broad downregulation of hsa_circ_0001368 in GC tissues and cells, which correlates with a worse prognosis in GC patients. Functional experiments suggested that the knockdown of hsa_circ_0001368 promoted cell viability and motility by cell proliferation and invasion assays. In addition, the knockdown of hsa_circ_0001368 led to accelerated tumor growth in vivo. Mechanically, we demonstrated that hsa_circ_0001368 served as a competing endogenous RNA (ceRNA) to sponge miR-6506–5p. Subsequently, FOXO3 may act as the functional target of miR-6506–5p, and the knockdown of hsa_circ_0001368 decreased the expression of the tumor-suppressive gene FOXO3. Taken together, our study revealed that hsa_circ_0001368 plays a tumor-suppression role in GC via the miR-6506–5p/FOXO3 axis and may serve as a potential target for GC therapy. •In this study, we performed high-throughput circRNA microarray assays using gastric cancer (GC) patient samples to investigate the potential involvement of circRNAs in GC, and we revealed that lower levels of circRNA hsa_circ_0001368 were expressed in GC tissues than in normal adjacent tissues.•Functionally, knockdown of hsa_circ_0001368 dramatically promoted the ability of GC cells to proliferate and invasion in vitro and in vivo.•Regarding the mechanism, we found that hsa_circ_0001368 was mainly observed in the cytoplasm and was capable of sponging miR-6506–5p to increase the expression of the tumor-suppressive gene FOXO3.•Our study first revealed a novel signaling pathway of hsa_circ_0001368/miR-6506-5p/FOXO3 involved in GC progression.