Details

Autor(en) / Beteiligte

• Hou, Gui-Ge
• Wang, Chun-Hua
• Sun, Ju-Feng
• Yang, Mei-Zi
• Lin, Dong
• Li, Hong-Juan

Titel

Rhodamine-based “turn-on” fluorescent probe with high selectivity for Fe2+ imaging in living cells

Ist Teil von

• Biochemical and biophysical research communications, 2013-10, Vol.439 (4), p.459-463

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2013

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A rhodamine-based “turn-on” fluorescent probe 1 was synthesized. 1 displays high selectivity toward Fe2+ without interfering by other transition metal ions. And 1 exhibits a stable response for Fe2+ over a concentration range from 2μM to 24μM. Their fluorescent enhancement is observed in the presence of Fe2+ because of the ring-open interactions of spirocyclic. In addition, confocal laser scanning microscopy experiments have proven that probe can be used to monitor Fe2+ in living cells. •A rhodamine-based “turn-on” fluorescent probe was synthesized with high yield.•Probe displays high selectivity for Fe2+ and a stable response range of 2–24μM.•The “turn-on” fluorescence is caused by open-cyclic mechanism of rhodamine.•Probe shows a good application to monitor Fe2+ in living cells by bioimaging. A rhodamine-based “turn-on” fluorescent probe 1 was synthesized with high yield. The recognizing behavior displays high selectivity of 1 toward Fe2+ with a 2:1 complex, and 1 exhibits a stable response for Fe2+ over a concentration range from 2μM to 24μM. Most importantly, probe is hardly interfered by other transition metal ions. Their fluorescent enhancement is observed in the presence of Fe2+ because of the ring-open interactions of spirocyclic. All measurements are made in PBS buffer environments simulating biological conditions to make them suitable candidates for fluorescent labeling of biological systems. Confocal laser scanning microscopy experiments have proven that probe can be used to monitor Fe2+ in living cells.