Details

Autor(en) / Beteiligte

• Gorny, Xenia
• Säring, Paula
• Bergado Acosta, Jorge R.
• Kahl, Evelyn
• Kolodziejczyk, Malgorzata H.
• Cammann, Clemens
• Wernecke, Kerstin E.A.
• Mayer, Dana
• Landgraf, Peter
• Seifert, Ulrike
• Dieterich, Daniela C.
• Fendt, Markus

Titel

Deficiency of the immunoproteasome subunit β5i/LMP7 supports the anxiogenic effects of mild stress and facilitates cued fear memory in mice

Ist Teil von

• Brain, behavior, and immunity, 2019-08, Vol.80, p.35-43

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• •Mice deficient of β5i/LMP7 display normal locomotor and exploratory activity in basic behavioral paradigms.•There is no effect of β5i/LMP7 deficiency on anxiety behavior in general.•β5i/LMP7-deficiency does not affect predator odor-induced defensive behavior, startle magnitudes and prepulse inhibition.•Depletion of β5i/LMP7 leads to increased anxiety after mild stress.•β5i/LMP7-deficient mice show no differences in contextual fear but increased cued fear conditioning compared to wildtype littermates. Proteolysis as mediated by one of the major cellular protein degradation pathways, the ubiquitin–proteasome system (UPS), plays an essential role in learning and memory formation. However, the functional relevance of immunoproteasomes in the healthy brain and especially their impact on normal brain function including processes of learning and memory has not been investigated so far. In the present study, we analyzed the phenotypic effects of an impaired immunoproteasome formation using a β5i/LMP7-deficient mouse model in different behavioral paradigms focusing on locomotor activity, exploratory behavior, innate anxiety, startle response, prepulse inhibition, as well as fear and safety conditioning. Overall, our results demonstrate no strong effects of constitutive β5i/LMP7-deficiency on gross locomotor abilities and anxiety-related behavior in general. However, β5i/LMP7-deficient mice expressed more anxiety after mild stress and increased cued fear after fear conditioning. These findings indicate that the basal proper formation of immunoproteasomes and/or at least the expression of β5i/LMP7 in healthy mice seem to be involved in the regulation of anxiety and cued fear levels.