Details

Autor(en) / Beteiligte

• dos Santos, G.A.
• Thomé, C.H.
• Ferreira, G.A.
• Yoneda, J.S.
• Nobre, T.M.
• Daghastanli, K.R.P.
• Scheucher, P.S.
• Gimenes-Teixeira, H.L.
• Constantino, M.G.
• de Oliveira, K.T.
• Faça, V.M.
• Falcão, R.P.
• Greene, L.J.
• Rego, E.M.
• Ciancaglini, P.

Titel

Interaction of 10-(octyloxy) decyl-2-(trimethylammonium) ethyl phosphate with mimetic membranes and cytotoxic effect on leukemic cells

Ist Teil von

• Biochimica et biophysica acta, 2010-09, Vol.1798 (9), p.1714-1723

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2010

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• 10-(Octyloxy) decyl-2-(trimethylammonium) ethyl phosphate (ODPC) is an alkylphospholipid that can interact with cell membranes because of its amphiphilic character. We describe here the interaction of ODPC with liposomes and its toxicity to leukemic cells with an ED-50 of 5.4, 5.6 and 2.9 μM for 72 h of treatment for inhibition of proliferation of NB4, U937 and K562 cell lines, respectively, and lack of toxicity to normal hematopoietic progenitor cells at concentrations up to 25 μM. The ED-50 for the non-malignant HEK-293 and primary human umbilical vein endothelial cells (HUVEC) was 63.4 and 60.7 μM, respectively. The critical micellar concentration (CMC) of ODPC was 200 μM. Dynamic light scattering indicated that dipalmitoylphosphatidylcholine (DPPC) liposome size was affected only above the CMC of ODPC. Differential calorimetric scanning (DCS) of liposomes indicated a critical transition temperature ( T c) of 41.5 °C and an enthalpy (∆H) variation of 7.3 kcal mol − 1. The presence of 25 μM ODPC decreased T c and ∆H to 39.3 °C and 4.7 kcal mol − 1, respectively. ODPC at 250 μM destabilized the liposomes (36.3 °C, 0.46 kcal mol − 1). Kinetics of 5(6)-carboxyfluorescein (CF) leakage from different liposome systems indicated that the rate and extent of CF release depended on liposome composition and ODPC concentration and that above the CMC it was instantaneous. Overall, the data indicate that ODPC acts on in vitro membrane systems and leukemia cell lines at concentrations below its CMC, suggesting that it does not act as a detergent and that this effect is dependent on membrane composition.