Details

Autor(en) / Beteiligte

• de Oliveira, Crislaine Palmeira Barbosa
• Lemos, Carlos Henrique da Paixão
• Vidal, Luiz Vitor Oliveira
• Couto, Ricardo David
• Pereira, Denise Soledade Peixoto
• Copatti, Carlos Eduardo

Titel

Anaesthesia with eugenol in hybrid Amazon catfish (Pseudoplatystoma reticulatum × Leiarius marmoratus) handling: Biochemical and haematological responses

Ist Teil von

• Aquaculture, 2019-02, Vol.501, p.255-259

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• This study aimed to evaluate the effectiveness of eugenol for induction and anaesthesia recovery in hybrid Amazon catfish juveniles and to verify its effects after handling stress. Juveniles were exposed to different concentrations of eugenol: 0 (control), 10, 20, 30, 40, 50 and 60 μL L−1 (equivalent to 10.6, 21.2, 31.8, 42.4, 53.0 or 63.6 mg L−1). The fish were divided into two groups: anaesthetised with 53.0 mg L−1 eugenol and non-anaesthetised. Biochemical and haematological variables showed different responses for eugenol in handling (0, 1 and 5 h). Eugenol was recommended for sedation and anaesthesia with 21.2 and 53.0 mg L−1, respectively. In control fish, 25 and 50% mortality occurred at zero and one hour after handling stress. Plasma cortisol levels were higher in control fish than anaesthetised fish (0 h). Plasma AST and albumin levels were higher in control fish when compared to anaesthetised fish (0 and 1 h, respectively). In control fish, plasma cortisol, glucose, total protein and albumin levels were significantly lower at five hours after handling stress. Thrombocyte and neutrophil values were lower in control fish when compared to anaesthetised fish (1 and 5 h, respectively). The use of 53.0 mg L−1 eugenol is indicated as an anaesthetic for Amazon catfish and reduces post-handling stress by reducing mortality, improving biochemical responses and promoting increased immune system. •This study provides information about eugenol as an anaesthetic and as a stress reducer in Amazon catfish.•In the non-anaesthetised fish, mortality was recorded after handling stress.•Fish anaesthetised with 50 μL L−1 (= 53 mg L−1) eugenol achieved lowest plasma cortisol after handling.•The highest thrombocytes and neutrophils values were observed in anaesthetised fish.