Details

Autor(en) / Beteiligte

• Caufour, Philippe
• Le Grand, Roger
• Chéret, Arnaud
• Neildez, Olivier
• Thiébot, Hugues
• Théodoro, Frédéric
• Boson, Bertrand
• Vaslin, Bruno
• Venet, Alain
• Dormont, Dominique

Titel

Longitudinal analysis of CD8 + T-cell phenotype and IL-7, IL-15 and IL-16 mRNA expressionin different tissues during primary simian immunodeficiency virus infection

Ist Teil von

• Microbes and infection, 2001-03, Vol.3 (3), p.181-191

Ort / Verlag

Elsevier SAS

Erscheinungsjahr

2001

Link zum Volltext

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Infection of macaques with pathogenic isolates of simian immunodeficiency virus (SIV) represents a useful model of HIV infection that offers the unique opportunity to investigate the very early modifications that affect CD8 + T-lymphocyte subsets and related cytokines during lentiviral infection. Herein, three cynomolgus macaques were inoculated intravenously with a pathogenic isolate of SIVmac 251. In fresh isolated mononuclear cells from blood, lymph node and bronchoalveolar lavage, we analyzed changes in the phenotype of CD8 + T cells and we used reverse transcription-PCR to monitor the expression of IL-7, IL-15 and IL-16 mRNA. We demonstrated that an expansion of CD8 +CD28 – T cells occurs from the third week of infection on in the peripheral blood and in the lung, whereas CD8 +CD28 + T cells expand in the lymph nodes. Concomitantly, we evidenced mRNA modulations in IL-16, IL-15 and IL-7 expression in the three compartments studied. The containment of systemic viral replication was associated with an overexpression of IL-16 mRNA in the lung and in the peripheral blood. Given the immunomodulatory properties of IL-15 and IL-7 and the potential antiviral ability of IL-16, these perturbations could have important implications in early viral dissemination and HIV immunopathogenesis.