Details

Autor(en) / Beteiligte

• Thieme, D
• Grosse, J
• Lang, R
• Mueller, R.K
• Wahl, A

Titel

Screening, confirmation and quantitation of diuretics in urine for doping control analysis by high-performance liquid chromatography–atmospheric pressure ionisation tandem mass spectrometry

Ist Teil von

• Journal of chromatography. Biomedical applications, 2001-06, Vol.757 (1), p.49-57

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2001

Quelle

EZB-NALI5-00465 Elsevier Archive NL

Beschreibungen/Notizen

• A sensitive, selective, robust and fast method to identify 32 diuretics and masking agents in urine is described. The analytical procedure is reduced to a single XAD extraction step for sample preparation, followed by reversed-phase liquid chromatography in combination with atmospheric pressure ionisation/tandem mass spectrometry. This technique is, after minor modifications, suitable for screening analyses and confirmation of identity as well as quantitation of diuretics. Considerations relating to the stability and metabolism of the compounds are given if relevant for routine screening analyses.