Details

Autor(en) / Beteiligte

• Leeb, Tosso
• Kriegesmann, Bernd
• Baumgartner, Bernhard G
• Klett, Christine
• Yerle, Martine
• Hameister, Horst
• Brenig, Bertram

Titel

Molecular cloning of the porcine β-1,2- N-acetylglucosaminyltransferase II gene and assignment to chromosome 1q23-q27

Ist Teil von

• Biochimica et biophysica acta, 1997-10, Vol.1336 (3), p.361-366

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

1997

Link zum Volltext

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Glycosyltransferases play an important role in the synthesis of glycoproteins. Here we report the isolation of a brain cDNA coding for 89% of the porcine UDP- N-acetylglucosamine: α-6- d-mannoside β-1,2- N-acetylglucosaminyltransferase II (EC 2.4.1.143) (GnTII). The cDNA was used for screening a genomic liver DNA library and isolation of a recombinant lambda FIX II phage containing the complete porcine GnTII gene and upstream and downstream sequences. The β-1,2- N-acetylglucosaminyltransferase II gene harbours a single exon with an open reading frame of 1338 bp coding for a 446 amino acid protein with a calculated molecular mass of 51.1 kDa. The promoter of the GnTII gene is lacking a TATA-box and shows variable transcription start sites. In the 3′-untranslated region a polymorphic polyadenosine stretch was detected. The porcine GnTII gene contains four polyadenylation sites. PCR analysis of a porcine-rodent hybrid cell panel revealed the chromosomal location of the GnTII gene on SSC 1q23-q27. The mapping data of the cell panel were confirmed by fluorescence in situ hybridization (FISH) on metaphase chromosomes.