Details

Autor(en) / Beteiligte

• Merle Elloso, M
• Wallace, Marianne
• Manning, D.D
• Weidanz, William P

Titel

The effects of interleukin-15 on human γδ T cell responses to Plasmodium falciparum in vitro

Ist Teil von

• Immunology letters, 1998-12, Vol.64 (2), p.125-132

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

1998

Link zum Volltext

Quelle

EZB-NALI5-00465 Elsevier Archive NL

Beschreibungen/Notizen

• We observed that the γ δ T cell subset expands when human peripheral blood mononuclear cells (PBMC) from malaria-naive donors are cultured with Plasmodium falciparum lysate in the presence of IL-2 or IL-15, cytokines that utilize two common IL-2 receptor subunits. IL-15 induced the expansion of the γ δ T cell subset at all levels tested, whereas IL-2 was not stimulatory at high levels. Flow cytometric analysis of apoptosis using the TUNEL assay indicated that the percentage and absolute number of γ δ T cells undergoing apoptosis were greater in cultures stimulated with antigen and IL-2 than in cultures stimulated with either antigen and IL-15 or control erythrocyte lysate and IL-2. The ability of IL-15 to enhance γ δ T cell function was also assessed; the results suggest that IL-15 can function with IL-2 to enhance the capacity of γ δ T cells to inhibit parasite replication. Together these data indicate that IL-2 and IL-15, which both bind to IL-2R β and IL-2R γc, enhance γ δ T cell function, but they appear to have different effects on proliferation and survival.