Details

Autor(en) / Beteiligte

• RUDRAMMAJI, L. M. S
• GOWDA, T. V

Titel

Purification and characterization of three acidic, cytotoxic phospholipases A2 from Indian cobra (Naja Naja Naja) venom

Ist Teil von

• Toxicon (Oxford), 1998-06, Vol.36 (6), p.921-932

Ort / Verlag

Oxford: Elsevier Science

Erscheinungsjahr

1998

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Three acidic phospholipases A2 (NN-I2c-PLA2, NN-I2d-PLA2 and NN-I2c-PLA2) were purified by successive chromatography of Indian cobra (Naja naja naja) venom on CM-Sephadex C-25, Sephadex G-50 and QAE Sephadex A-25 columns. They have molecular weights of 13,000-14,500 by sodium dodecyl sulphate polyacrylamide gel electrophoresis. They showed tryptophan specific fluorescence emission spectra (approximately 345 nm). All the three phospholipases A2 were enzymatically highly active with specific activities 9-17 micromol min(-1) mg(-1). They were non-lethal to mice when injected intraperitoneally in doses up to 10 mg kg(-1) body weight. They induced edema in mouse foot pads and were cytotoxic to Ehrlich ascites tumour cells. They did not exhibit direct haemolytic and anticoagulant activities.