Details

Autor(en) / Beteiligte

• Iyer, Radhakrishnan P.
• Yu, Dong
• Habus, Ivan
• Ho, Nan-Hui
• Johnson, Suzanne
• Devlin, Theresa
• Jiang, Zhiwei
• Zhou, Wen
• Xie, Jin
• Agrawal, Sudhir

Titel

N-pent-4-enoyl ( PNT) group as a universal nucleobase protector: Applications in the rapid and facile synthesis of oligonucleotides, analogs, and conjugates

Ist Teil von

• Tetrahedron, 1997-02, Vol.53 (8), p.2731-2750

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

1997

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Rapid synthesis of phosphoric diester and phosphorothioate oligonucleotides in 1 micromol to 1 millimol scale has been achieved using PNT nucleoside phosphoramidites. Furthermore, facile synthesis of mixed backbone oligonucleotides (MBOs) as antisense agents can be carried out using PNT nucleoside phosphoramidites in conjunction with PNT nucleoside H-phosphonates and PNT nucleoside phosphonamidites. The versatility of the PNT group is further demonstrated by its use in the preparation of bioreversible oligonucleotide conjugates. Multiple deprotection strategies allow rapid access to oligonucleotides, analogs, and conjugates.