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1. The effects of temperature acclimation at 10, 20 and 30°C on the concentration and activity of the mixed function oxidase system in bluegill are as follows.
2. Liver weight/body weight varied inversely with temperature.
3. Significant (
P < 0.05) differences in concentration of cytochrome P-450 of hepatic microsomes were seen and varied inversely with temperature.
4. Benzo(
a)pyrene hydroxylase activity tested
in vitro at incubation temperatures of 10, 20 and 30°C showed significant differences in
K
m, but no differences in
V
max.
5. SDS polyacrylamide gel electrophoresis revealed some quantitative differences in cytochrome P-450 isozymes between groups.