Details

Autor(en) / Beteiligte

• Hublart, M
• Mendonça-Previato, L
• Boutignon, F
• Huet-Duvillier, G
• Degand, P

Titel

Evidence of myristylated disulfide-linked dimer of variant surface glycoprotein of Trypanosoma brucei-brucei

Ist Teil von

• Comparative biochemistry and physiology. B, Comparative biochemistry, 1989, Vol.92 (4), p.705-710

Ort / Verlag

England

Erscheinungsjahr

1989

Quelle

MEDLINE

Beschreibungen/Notizen

• 1. Variant surface glycoprotein (VSGs) of Trypanosoma brucei-brucei may exist as a disulfide-linked dimer in both forms: myristylated (mfVSG) and non-myristylated (sVSG), as judge by fluorography and immunoblotting of SDS-PAGE under non-reducing conditions. 2. The dimeric VSG form is labeled with [3H]-myristic acid in our incorporation conditions. 3. AnTat 1.1 trypanosomes preincubated with tunicamycin and incubated with [3H]-myristic acid synthesized a labeled molecule that has an apparent molecular weight slightly smaller than the native form, and that also corresponds to a disulfide-linked dimer.