Details

Autor(en) / Beteiligte

• Bates, George W.
• Goldsmith, Mary Helen M.
• Goldsmith, Timothy H.

Titel

Membranes of oat cells: The inverse relation between voltage and resistance is not due to H + pumps

Ist Teil von

• Plant science letters, 1983-01, Vol.30 (3), p.279-284

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

1983

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Intracellular impalements of oat coleoptile cells showing the highest input resistances (R) are always associated with less negative membrane voltages (V), whereas impalements with lower resistances display more negative voltages. Both fusicoccin (FC) and high external pH cause oat cells to hyperpolarize as much as 45 mV without altering input resistance. Furthermore, the extent of hyperpolarization is the same whether the initial R of the implements is low (< 5 MΩ) or high (⩾ 15 MΩ) . The characteristics relationship between R and membrane potential is therefore not the result of a heterogeneous population of cells displaying varying degrees of electrogenic proton pumping but reflects instead, as previously proposed, cells with two compartments, with the higher resistance barrier around the vacuole. The hyperpolarizing effects of FC and high external pH occur at the plasma membrane.