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l-Sorbose production from glucose and fructose using Zymomonas mobilis and Gluconobacter suboxydans in a two-stage fed-batch reactor
Ist Teil von
Process biochemistry (1991), 1994, Vol.29 (4), p.277-283
Ort / Verlag
Oxford: Elsevier Ltd
Erscheinungsjahr
1994
Link zum Volltext
Quelle
Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
Beschreibungen/Notizen
A biological conversion of
L-sorbose from the mixture of glucose and fructose was studied.
D-Sorbitol was produced efficiently from fructose by permeabilized
Zymomonas mobilis cells and then converted to
L-sorbose by
Gluconobacter suboxydans. The process for the production of
L-sorbose from
D-sorbitol by the resting cells of
G. suboxydans was also optimized with respect to the substrate concentration, oxygen tension and cell density of the reaction medium. Gluconate produced from glucose by
Z. mobilis seemed to act as an inhibitor in the conversion reaction of
D-sorbitol into
L-sorbose by
G. suboxydans. A two-stage fed-batch process, therefore, was developed to minimize the inhibitory effect of gluconate on the conversion reaction.