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High-sensitivity laser-induced fluorescence detection of native proteins in capillary electrophoresis
Ist Teil von
Journal of Chromatography A, 1992-03, Vol.595 (1), p.319-325
Ort / Verlag
Amsterdam: Elsevier B.V
Erscheinungsjahr
1992
Link zum Volltext
Quelle
Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
Beschreibungen/Notizen
A highly sensitive laser-induced (LIF) detection scheme for native, tryptophan- or tyrosine-containing proteins in capillary electrophoresis (CE) has been demonstrated. The 275.4 nm line from an argon-ion laser is used to excite native protein fluorescence. A limit of detection (LOD) (
S/N = 2)of 1 · 10
−10
M for conalbumin represents a 140-fold improvement over earlier reports. With stacking at injection, the LOD is 3 · 10
−12
M. Linear dynamic ranges of at least 5 and 4 orders of magnitude for, respectively, tryptophan and bovine serum albumin are found. The practical performance and blueprint of an easily constructed, rugged, compact and user-friendly LIF detector for CE are shown.