Details

Autor(en) / Beteiligte

• Cohen, Smadar
• Ban˜ó, M. Carmen
• Chow, Marie
• Langer, Robert

Titel

Lipid-alginate interactions render changes in phospholipid bilayer permeability

Ist Teil von

• Biochimica et biophysica acta, 1991-03, Vol.1063 (1), p.95-102

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

1991

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Lipid vesicles, e.g. liposomes, generally release their contents in a continuous manner. However, when these vesicles are entrapped in Ca-alginate and coated with poly( l-lysine), they release their contents in an unusual fashion, in ‘bursts’. Molecular-level studies indicated that lipid-alginate interactions are responsible for changes in the barrier properties of lipid vesicles. Differential scanning calorimetry revealed that exposure of liposomes to alginate resulted in a 4-fold reduction in the phase transition enthalpy, with no change in the melting temperature. Size-exclusion chromatography of liposomes-in-alginate gave an additional liposomal peak with a smaller elution volume. These studies suggested that alginate is inserted into the lipid bilayer of vesicles. Lipid-alginate interactions were highly dependent on phospholipid head group charge and the phase transition temperature of the phospholipid. Based on these interactions, a mechanism to explain the ‘burst’ from these entrapped liposomes is suggested.