Biochimica et biophysica acta, 1987-08, Vol.902 (1), p.93-100
1987
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- Autor(en) / Beteiligte
- Titel
- Decreased transport of d-glucose and l-alanine across brush-border membrane vesicles from small intestine of rats treated with mitomycin C
- Ist Teil von
- Biochimica et biophysica acta, 1987-08, Vol.902 (1), p.93-100
- Ort / Verlag
- Netherlands: Elsevier B.V
- Erscheinungsjahr
- 1987
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- To elucidate the mechanisms underlying the dysfunctions of intestinal absorption induced by antitumor drugs, the effect of pretreatment with mitomycin C on sodium gradient-dependent d-glucose and l-alanine transports was studied in rat brush-border membrane vesicles. 24, 48, 96, or 120 h following a single intravenous injection of mitomycin C, brush-border membrane vesicles were prepared from rat small-intestines. The uptake of d-glucose and l-alanine was shown to be Na + gradient-dependent even in the case of vesicles obtained from mitomycin C-treated rats, but uptake rates measured at 15 s and magnitude of overshooting effect in uptake of both solutes were decreased in vesicles maximally from 48 h mitomycin C-treated rats. The rate of d-glucose uptake calculated at 15 s recovered to the control level in vesicles prepared at 96 h and 120 h after mitomycin C-treatment, indicating that the effect of mitomycin C on Na + gradient-dependent d-glucose transport would be fully reversible. Tracer exchange experiments under Na + and d-glucose equilibrated conditions indicated that the Na +/ d-glucose transporters were similarly operative in the vesicles from control and 48 h mitomycin C-treated rats. Rates of 22Na + uptake measured at 15 s in vesicles from 48 h mitomycin C-treated rats, however, were increased. The increased permeability to Na + might bring about a more rapid dissipation of the Na + gradient in these vesicles and this would secondarily cause the decrease in Na +-dependent d-glucose uptake in vesicles from mitomycin C-treated rats.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0005-2736, 0006-3002eISSN: 1879-2642, 1878-2434DOI: 10.1016/0005-2736(87)90139-8Titel-ID: cdi_crossref_primary_10_1016_0005_2736_87_90139_8
- Format
- –
- Schlagworte
- Alanine - metabolism, Alanine transport, Animals, Biological Transport - drug effects, Brush-border membrane, Cell Membrane Permeability, Cotransporter, Na +/ d-glucose, Fluorescence Polarization, Glucose - metabolism, Glucose transport, Intestine, Small - drug effects, Intestine, Small - metabolism, Kinetics, Liposomes - metabolism, Male, Membrane Fluidity - drug effects, Microvilli - drug effects, Microvilli - metabolism, Mitomycin, Mitomycin C, Mitomycins - pharmacology, Monosaccharide Transport Proteins - metabolism, Rat small intestine, Rats, Rats, Inbred Strains, Sodium - metabolism, Sodium gradient