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New design for multi-crystal data collection at SSRF
Ist Teil von
Nuclear science and techniques, 2018-02, Vol.29 (2), p.48-56, Article 21
Ort / Verlag
Singapore: Springer Singapore
Erscheinungsjahr
2018
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Data collection with microcrystals at synchrotron radiation facilities is challenging because it is difficult to harvest and locate microcrystals.Moreover,microcrystals are sensitive to radiation damage;thus,typically,a complete data set cannot be obtained with a single microcrystal.Herein,we report a new method for data collection with multiple microcrystals having a crystal size ranging from1to30lm.This method is suitable for not only low-temperature(100K)data collection but also room-temperature data collection.Thin Kapton membranes were used to capture multiple crystals simultaneously.The microcrystals were visible under an optical microscope and easily located because the membrane was transparent and sufficiently thin.The film was fixed to a bracket that was prepared using a three-dimensional printer.The bracket was fixed on a magnetic base via screwing and employed by the goniometer system for data collection.Multiple data sets of fatty acid-binding protein4(FABP4)and lysozyme microcrystals were collected using this novel designed device.Finally,the structures of protein FABP4and lysozyme were obtained from these data via the molecule replacement method.The data statistics reveal that this method provides a comparable result to traditional methods such as a nylon loop.