Details

Autor(en) / Beteiligte

• Kayukawa, Takumi
• Tetsuro Shinoda

Titel

Functional characterization of two paralogous JH receptors, methoprene-tolerant 1 and 2, in the silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Ist Teil von

• Applied entomology and zoology, 2015-08, Vol.50 (3), p.383-391

Ort / Verlag

Tokyo: Springer Japan

Erscheinungsjahr

2015

Quelle

SpringerNature Journals

Beschreibungen/Notizen

• Methoprene-tolerant (Met) is a bHLH-PAS transcription factor that functions as a core component of juvenile hormone (JH) receptors in insects. The genome of the silkworm, Bombyx mori (L.), encodes two Met paralogs, BmMet1 and BmMet2. We have previously proposed that BmMet2 forms a functional complex with steroid receptor co-activator (BmSRC) in the presence of JH and induces Krüppel-homolog 1 (BmKr-h1). In this study, we characterized BmMet2 domains and examined the role of BmMet1. A series of reporter and two-hybrid assays on BmMet2 revealed that the bHLH domain played a critical role in the binding to the JH response element of BmKr-h1 (kJHRE), the PAS-A in the transcriptional activation, and the PAS-B in the JH recognition. Furthermore, we confirmed that the BmMet2/BmSRC complex recognizes the core kJHRE (GGCCTCCACGTG). BmMet1 was found to interact with BmSRC and weakly activated the kJHRE reporter in a JH-dependent manner. The BmMet1 transcript level was much higher than the BmMet2 transcript level in the epidermis during the larval and pupal stages. By contrast, the BmMet2 transcript was hardly detected during the larval stage, but was abundant in the adult stage. These results suggest that BmMet1 is involved in the JH-dependent induction of BmKr-h1 in larvae, whereas BmMet2 is involved in adults.