Details

Autor(en) / Beteiligte

• Tiwari, K. N
• Tiwari, V
• Singh, J
• Singh, B. D
• Ahuja, P

Titel

Synergistic effect of trimethoprim and bavistin for micropropagation of Bacopa monniera

Ist Teil von

• Biologia plantarum, 2012-03, Vol.56 (1), p.177-180

Ort / Verlag

Dordrecht: Springer-Verlag

Erscheinungsjahr

2012

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• A micropropagation protocol for Bacopa monniera (L.) Wettst., a medicinally important plant, has been developed. Direct organogenesis without callus formation was induced by culturing node, internode and leaf explants on growth regulator free Murashige and Skoog (MS) medium. MS medium supplemented with an antibiotic trimethoprim (TMP) and a fungicide bavistin (BVN) produced axillary shoots from node and adventitious shoot buds on the surface of all explants. The combination of 200 mg dm−3 TMP and 200 mg dm−3 BVN induced the optimum frequency of shoot formation as well as shoot number. Presence of both TMP and BVN induced multiple axillary shoot formation from the nodal segments and this ability was maintained for four subcultures.