Details

Autor(en) / Beteiligte

• Alizadeh, M.,Indian Agricultural Research Inst., New Delhi (India). Div. of Fruits and Horticultural Technology
• Singh, S.K.,Indian Agricultural Research Inst., New Delhi (India). Div. of Fruits and Horticultural Technology
• Patel, V.B.,Indian Agricultural Research Inst., New Delhi (India). Div. of Fruits and Horticultural Technology
• Bhattacharya, R.C.,IARI, New Delhi (India). National Research Centre on Plant Biotechnology
• Yadav, B.P.,IARI, New Delhi (India). National Research Centre on Plant Biotechnology

Titel

In vitro responses of grape rootstocks to NaCl

Ist Teil von

• Biologia plantarum, 2010-06, Vol.54 (2), p.381-385

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2010

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• An investigation was undertaken to adjudge the in vitro salt tolerance and biochemical changes due to NaCl on four grape rootstocks (Dogridge, SO4, H-144 and 3309C). The in vitro two-node micro-cuttings from the established cultures were sub-cultured on rooting medium comprising Murashige and Skoog basal medium supplemented with 0.5 microM indolebutyric acid (IBA), 200 mg/cubic dm activated charcoal and different NaCl concentrations ranging from 0 to 125 mM. The surviving and proliferated cultures were further sub-cultured four times on respective media. Dogridge and H-144 tolerated 125 and 100 mM NaCl, respectively, while SO4 and 3309C survived only up to 75 mM NaCl. Contents of proteins, proline, K+ and Na+ in tissue increased in all genotypes due to NaCl supplementation, while contents of chlorophyll and total soluble sugars declined. Higher K+/Na+ ratio was registered in Dogridge and H-144 than in SO4 and 3309C. The relative NaCl tolerance for different grape rootstocks under study could be ranked as Dogridge more than H-144 more than SO4 and 3309C.