Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Electrochemical myoglobin biosensor based on carbon ionic liquid electrode modified with Fe3O4@SiO2 microsphere
Ist Teil von
Journal of solid state electrochemistry, 2014, Vol.18 (1), p.207-213
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2014
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
In this paper, a Fe
3
O
4
@SiO
2
core-shell structure microsphere was synthesized and used to investigate the direct electron transfer of myoglobin (Mb) with a 1-butylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) as the substrate electrode. The mixture of Mb and Fe
3
O
4
@SiO
2
microsphere could form an organic–inorganic composite, which was immobilized on the surface of CILE with a chitosan (CS) film. Cyclic voltammetric experiments indicated that a pair of well-defined quasi-reversible redox peaks appeared on CS/Mb-Fe
3
O
4
@SiO
2
/CILE with the formal peak potential (
E
0′
) located at −0.31 V (vs. saturated calomel electrode), which was corresponded to the electroactive center of Mb heme Fe(III)/Fe(II) redox couples. Direct electrochemical behaviors of Mb in CS-Fe
3
O
4
@SiO
2
composite film were carefully investigated with the electrochemical parameters calculated. The CS/Mb-Fe
3
O
4
@SiO
2
/CILE showed good electrocatalytic behaviors to the reduction of trichloroacetic acid in the concentration range from 0.2 to 11.0 mmol L
−1
with the detection limit of 0.18 mmol L
−1
(3σ). Based on CS/Mb-Fe
3
O
4
@SiO
2
/CILE, a new third-generation reagentless electrochemical biosensor was constructed with higher sensitivity and reproducibility.